Project

Part:BBa_I3425:Design

Designed by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)   Group: Antiquity   (2004-01-28)


Test: Switch aspartate excretion (I3410.I3200.I3411.E0432.I3100.I3400.I3101.I3400.I3102.I3401)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 551
    Illegal BglII site found at 942
    Illegal BglII site found at 2407
    Illegal BglII site found at 5432
    Illegal BglII site found at 6285
    Illegal BglII site found at 8184
    Illegal BamHI site found at 9885
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1834
    Illegal AgeI site found at 1363
    Illegal AgeI site found at 2215
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1874
    Illegal SapI.rc site found at 715
    Illegal SapI.rc site found at 6449
    Illegal SapI.rc site found at 8348


Design Notes

The layout of the switch mimics the layout of the proposed Polkadorks E. colibrator (I3000). Two switch outputs are insulated by appearing first. Hk022cI is attached to the AspA attractant sender (I3410.I3200). Lambda cI is attached to the YFP reporter (I3411.E0432). The three individually controlled trigger inputs follow the outputs in the layout. Lac trigger controls one hk022cI input (I3100.I3400), and TetR trigger controls the other (I3101.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, AraC trigger controls the lambda cI input (I3102.I3401).



Source

References